Journal: ACS Omega

Article Title: Combination of UPLC–Q-TOF/MS and Network Pharmacology to Reveal the Mechanism of Qizhen Decoction in the Treatment of Colon Cancer

doi: 10.1021/acsomega.1c01183

Figure Lengend Snippet: (A) After LoVo cells were treated with LY294002 or different concentrations of QZD, the protein expression of P-Akt and Akt was detected by western blotting. (B) Quantitative histogram of P-Akt/Akt of LoVo cells treated with LY294002h or different concentrations of QZD. (C) MTT assay was used to detect the cell viability of LoVo cells treated with different concentrations of QZD or LY294002. (D) Cell wound scratch test was used to detect the quantitative histogram of the healing rate of LoVo cells treated with different concentrations of QZD or LY294002. (E) Cell wound scratch test was used to detect the representative diagram of cell migration ability of LoVo cells treated with different concentrations of QZD or LY294002 (40×).

Article Snippet: Human colon cancer cell line LoVo (ATCC); P-Akt antibody, Akt antibody, LY294002 (Selleck Company); β-actin antibody, goat antirabbit IgG (Abcam, U.K.); MTT (Solarbio, China), dimethyl sulfoxide (DMSO) (Sigma), Roswell Park Memorial Institute (RPMI) 1640 medium, fetal bovine serum (FBS) (Gibco), bicinchoninic acid (BCA) protein quantitative analysis kit (Solarbi, China), a fluorescence inversion microscope (Olympus, Japan), an enzyme labeling instrument (Thermo), an electrophoresis instrument (Bio-Rad), a transmembrane instrument (Bio-Rad), a chemiluminescence gel imaging system (Aplegen), a Waters Acquity UPLC Class I ultrahigh-performance liquid chromatograph, a Waters SYNAPT G2-Si Q-TOF/MS in situ ion exposure high-resolution mass spectrometer, a Waters Acquity UPLC BEH C 18 column (2.1 mm × 100 mm, 1.7 μm) were used.

Techniques: Expressing, Western Blot, MTT Assay, Migration